|
KMID : 0380420180420020047
|
|
Journal of Prventive Veterinary Medicine
2018 Volume.42 No. 2 p.47 ~ p.53
|
|
|
Immune modulatory and protective effects of Brucella abortus cellular antigens, arginase and elongation factor Ts, as subunit vaccines against murine brucellosi
|
|
Reyes Alisha Wehdnesday Bernardo
Arayan Lauren Togonon
Hop Huynh Tan
Huy Tran Xuan Ngoc
Son Vu Hai
Kim Suk
|
|
|
Abstract
|
|
|
|
We investigated the effects of two Brucella proteins expressed in a pMAL expression system, RocF and EF-Ts, as subunit vaccines on immune modulation and protective efficacy using a mouse model. Mice vaccinated with MBP-RocF and MBP-EF-Ts displayed increased production of TNF, IFN-��, MCP-1, IL-10 and IL-6, and TNF and MCP-1, respectively. Furthermore, mice vaccinated with MBP-EF-Ts showed decreased induction of IFN-�� and Th2-related cytokines, IL-10 and IL-6. Higher proportions of CD4+ and CD8+ T cells were observed in the blood of mice vaccinated with MBP-RocF than in the PBS-vaccinated group, although the increases were not significant. Furthermore, significantly reduced Brucella proliferation in the spleens of the MBP-RocF and MBP-EF-Ts groups were observed, but inflammation of these organs was not attenuated. Overall, these results indicate that RocF and EF-Ts could be potential subunit vaccine candidates against animal brucellosis.
|
|
|
KEYWORD
|
|
|
Brucella abortus, MBP, RocF, EF-Ts, cytokines
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|